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KMID : 0903519950380010001
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1995 Volume.38 No. 1 p.1 ~ p.6
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Continuous Prodcution of δ - Aminolevulinate from Immobilized Cells of Rhodocyclus gelatinosus KUP - 74
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Abstract
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An optimal condition for the continuous production of δ-aminolevulinate(ALA) was investigated using high concentrated resting cells of Rhodocyclus gelatinosus KUP-74. The increase of the amount of extracellular ALA versus the concentration of resting cells showed rectangular hyperbolic pattern until 20 ㎎ cells/㎖, but no further increase in the ALA amount by increasing its concentration was occurred. The highest yield of the extracellular ALA was observed after 3 hr of incubation of 1 ㎖ reaction system containing 20 ㎎ cells, 4 mM lewlinate and 5 mM L-glutamate. On the other hand, the immobilized cells prepared by Ca-alginate inclusive method needed to incubate for 6 hr with 6 mM levulinate and 10 mM L-glutamate to give maximal yield of the extracellular ALA. In addition, under these conditions the resulted continuous productivities of the ALA by immobilized cells and highly concentrated resting cells were appeared 50 percent decreases after incubations for 185 hr and 100 hr, respectively, and the method of the cells to be immobilized was more efficient to recover the extracellular ALA produced.
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